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fig. 1 —JOINN Biologics 1分快三计划软件下载
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| Subject | Characteristics | JOINN’s Analytical Methods |
|---|---|---|
| Spent Media | Expressions (titer) Amino Acids Vitamins | HPLC, biosensor (Octet) HPLC HPLC |
| Proteins | Concentration Integrity Heterogeneity Aggregation Charge variants Glycosylation Thermostability Binding activity Viscosity Turbidity | UV spectrometer CE-SDS, SDS-PAGE, MassSpec MassSpec (intact, subunit, peptide mapping) SEC-MALS, 96-well DLS, Non-reducing SDS Page Capillary isoelectric focusing (cIEF), HPLC-IEX Released glycan by HPLC, MassSpec for attached glycan DSF Octet, ELISA Visometer Vis spectrometer |
| Impurities | Endotoxin HCP Protein A Residual DNA | LAL ELISA ELISA qPCR |